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J. Biochem, 1987, Vol. 101, No. 4 1055-1058
© 1987 Japanese Biochemical Society

other

Guanine Nucleotides Stimulate Arachidonic Acid Release by Phospholipase A2 in Saponin-Permeabilized Human Platelets1

Shigeru NAKASHIMA, Toyohiko TOHMATSU, Hiroaki HATTORI, Akiyoshi SUGANUMA and Yoshinori NOZAWA

Department of Biochemistry, Gifu University School of Medicine, Gifu, Gifu 500

GTP or GTP{gamma}S alone caused low but significant liberation of arachidonic acid in saponin-permeabilized human platelets but not in intact platelets. GTP or GTP{gamma}S also enhanced thrombin-induced [3H]arachidonic acid release in permeabilized platelets. Inhibitors of the phospholipase C (neomycin)/diacylglycerol lipase (RHC 80267) pathway for arachidonate liberation did not reduce the [3H]arachidonic acid release. The loss of [3H]arachidonate radioactivity from phosphatidylcholine was almost equivalent to the increase in released [3H]arachidonic acid, suggesting the hydrolysis of phosphatidylcholine by phospholipase A2. The effect of GTP{gamma}S was greater at lower Ca2+ concentrations. These data indicate that the release of arachidonic acid by phospholipase A2 in saponin-treated platelets may be linked to a GTP-binding protein.

1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture and Science of Japan.


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