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J. Biochem, 1987, Vol. 101, No. 4 825-835
© 1987 Japanese Biochemical Society

other

A New Ganglioside Showing Choleragenoid-Binding Activity in Mouse Spleen1

Kyoko NAKAMURA*, Minoru SUZUKI*, Fuyuhiko INAGAKI**, Tamio YAMAKAWA* and Akemi SUZUKI*

*Metabolism Section Bunkyo-ku, Tokyo 113
**Department of Medical Chemistry, The Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113

A new ganglioside showing choleragenoid-binding activity was purified from mouse spleen and characterized. From the results of sugar-composition analysis, enzymatic hydrolysis, a permethylation study, 1H-NMR spectroscopy, and negative-ion fast atom bombardment mass spectrometry, the structure of the ganglioside was determined to be as follows:



This ganglioside contains a terminal tetrasaccharide structure identical with that of II3NeuGc{alpha}-Gg4Cer (GM1(NeuGc)). By means of a TLC-immunobinding assay and an enzyme-linked immunosorbent assay, the ganglioside was demonstrated to have almost the same choleragenoid-binding activity as GM1. Another ganglioside, that migrated faster than the new choleragenoid-binding ganglioside, was also purified from the same source material and identified as IV4GalNAcß, IV3NeuGc{alpha}-Gg4Cer (GalNAc-GM1b(NeuGc)). Since, in the previous study, we demonstrated the existence of IV3NeuGc{alpha}-Gg4Cer (GM1b(NeuGc)) in mouse spleen (Nakamura, K. et al. (1984) J. Biochem. 96, 949–957), the results of this study suggest that the new choleragenoid-binding ganglioside is synthesized from GM1b(NeuGc) through GalNAc-GM1b(NeuGc).

1This study was supported in part by grants from the Ministry of Education, Science and Culture of Japan, and Special Coordination Funds for Promoting Science and Technology from the Science and Technology Agency of Japan.


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