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J. Biochem, 1987, Vol. 101, No. 4 905-910
© 1987 Japanese Biochemical Society

other

Conversion of Androstenedione to 3ß-Hydroxy-5-Androsten-17-One and 3ß-Hydroxy-4-Androsten-17-One by the Testicular Microsomal Fraction of Sprague-Dawley Rats1

Masakazu TAKAHASHI*,2, Hiroshi INAO**, Bun-ichi TAMAOKI**,3 and Sumi NAGASE*

*Department of Chemistry, Sasaki Institute Chiyoda-ku, Tokyo 101
**National Institute of Radiological Sciences Chiba, Chiba 260

2 To whom correspondence should be addressed.

3Present address: Department of Radiopharmacology, Faculty of Pharmaceutical Sciences, Nagasaki, Nagasaki 852, Japan.

When androstenedione was incubated with testicular microsomes of Sprague-Dawley rats in the presence of reduced nicotinamide-adenine dinucleotide (NADH), unknown metabolites were produced, in addition to testosterone and 7{alpha}-hydroxyandro-stenedione The metabolites were identified as 3ß-hydroxy-4-androsten-17-one and 3ß-hydroxy-5-androsten-17-one (3:1) by biochemical and radiochemical methods. These results confirmed the occurrence of the reverse reactions from androstenedione to 3ß-hydroxy-5-androsten-17-one and 3ß-hydroxy-5-androsten-17-one catalyzed by the 3ß-hydroxysteroid dehydrogenase and 5-ene-4-ene isomerase in the microsomal fraction of Sprague-Dawley rat testes.

1This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.


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