Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by TSUJIMOTO, M.
Right arrow Articles by VILCEK, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by TSUJIMOTO, M.
Right arrow Articles by VILCEK, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1987, Vol. 101, No. 4 919-925
© 1987 Japanese Biochemical Society

other

Comparative Studies of the Biological Activities of Human Tumor Necrosis Factor and Its Derivatives

Masafumi TSUJIMOTO*, Shoji TANAKA*, Yayoi SAKURAGAWA*, Nobuo TSURUOKA*, Kyoko FUNAKOSHI*, Tomoko BUTSUGAN*, Hiroshi NAKAZATO*, Tatsuro NISHIHARA*, Teruhisa NOGUCHI* and Jan VILCEK**

*Suntory Institute for Biomedical Research Mishima, Osaka 618;
**Department of Microbiology, New York University Medical Center New York, NY 10016, U.S.A.

Biological activities of human tumor necrosis factor (TNF) and its derivatives were compared. In cytotoxicity assay with L929 cells, one derivative, designated as TNF(Asn), showed significantly lower activity than any other TNF examined. In binding assay, this derivative was also shown to have lower affinity for TNF receptors on L929 cells, suggesting that the cytotoxic activity of TNFs on L929 cells correlates with their affinity for receptors. We also found that the cytotoxic activity of TNF on A673 cells and its inhibitory effect on lipoprotein lipase were parallel with the cytotoxic activity on L929 cells, but the growth-enhancing activity on FS-4 cells and the cytotoxic activity on endothelial cells were not. It was also shown that TNF(Asn) had lower affinity than any other TNF for receptors on these target cells tested. These results suggested that there might be at least two types of cellular responses to TNF; one might correlate with the receptor-binding affinity of TNFs and the other not.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S.-S. Cha, J.-S. Kim, H.-S. Cho, N.-K. Shin, W. Jeong, H.-C. Shin, Y. J. Kim, J. H. Hahn, and B.-H. Oh
High Resolution Crystal Structure of a Human Tumor Necrosis Factor-alpha Mutant with Low Systemic Toxicity
J. Biol. Chem., January 23, 1998; 273(4): 2153 - 2160.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.