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J. Biochem, 1987, Vol. 101, No. 4 927-932
© 1987 Japanese Biochemical Society

other

Time-Dependent Differential Effects of Natural and Recombinant Murine Interferon-Gamma on Ornithine Decarboxylase Activity of Tumor Cells1

Lucia GANDINO*, Giorgio GRIBAUDO*, Pietro GHEZZI** and Santo LANDOLFO*,2

*Department of Microbiology, Medical School, University of Torino Torino 10126, Italy
**Istituto di Ricerche Farmacologiche "Mario Negri" Milano 20157, Italy

2To whom correspondence should be addressed at: Istituto di Microbiologia, Via Santena 9, Torino 10126. Italy.

Incubation of quiescent tumor cells with fetal calf serum induced ornithine decar-boxylase (ODCase) activity concomitantly with mitogenic stimulation. Pretreatment of cells with highly purified natural or recombinant murine interferon-gamma (MuIFN-{gamma}) for 5 h caused a dose-dependent increase of ODCase activity induced by fetal calf serum (FCS). Pretreatment of target cells with IFN-{gamma} for 5 h in absence of FCS stimulation did not induce ODCase activity. When pretreatment of cells with natural or recombinant MuIFN-{gamma} was prolonged for 18 h both ODCase activity and DNA synthesis induced by FCS were suppressed. By contrast when a mixture of MuIFN-{alpha} and -ß was used, ODCase activity was significantly suppressed after 5 h pretreatment compared to untreated controls. These results suggest that IFN-{gamma} exerts a differential effect on mitogen-stimulated events depending on the dose and the time of addition.

1This work was supported by grants from the Italian National Research Council (CNR, PF ‘Controllo Malattie da Infezione’ No. 86.01596.52 and PF ‘On-cologia’ No. 86.01596.44) and from Ministero Pubblica Istruzione (40% ).


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