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J. Biochem, 1987, Vol. 101, No. 4 933-938
© 1987 Japanese Biochemical Society

other

Transformation of Macrophages into Foam Cells In Vitro Induced by Cholesteryl Oleate Liquid Crystals

Masayasu ENOMOTO*, Keiji NAKAGAMI*, Shoji OHKUMA*,** and Tatsuya TAKANO*,**,1

*Research Development Corporation of Japan Bunkyo-ku, Tokyo 112
**Department of Microbiology and Molecular Pathology, Teikyo University Sagamiko, Kanagawa 199–01

1To whom correspondence should be addressed.

Transformation of macrophages into foam cells after the uptake of cholesteryl oleate anisotropic liquid crystals was studied. A new technique to enhance the uptake of the liquid crystals by macrophages using an inverted petri dish was developed. Uptake of lipid droplets was found to increase in parallel with the amount of liquid crystals in the medium. A lysosomal enzyme was shown (by using lysosomotropic chloroquine) to be involved in the hydrolysis of the liquid crystals. About 47 and 72% of the [3H]cholesterol in liquid crystal-laden cells had disappeared after chase for 24 and 48 h, respectively. Thus the 50% clearance time of the liquid crystals by the macrophages was about 24 h, which was longer than that of denatured lipo-protein. A possible model of transformation of macrophages to foam cells is discussed.


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