Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by HIRABAYASHI, J.
Right arrow Articles by KASAI, K.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HIRABAYASHI, J.
Right arrow Articles by KASAI, K.-i.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1987, Vol. 101, No. 4 987-995
© 1987 Japanese Biochemical Society

other

Further Characterization and Structural Studies on Human Placenta Lectin1

Jun HIRABAYASHI*, Hiroshi KAWASAKI**, Koichi SUZUKI** and Ken-ichi KASAI*

*Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199–01;
**Department of Biology, The Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113

The properties of a previously purified ß-galactoside-binding lectin of human pla-centa were studied in detail. Isoelectric focusing gave multiple bands around pH 4.9, although the lectin preparation was homogenous in SDS-polyacrylamide gel electrophoresis. High-performance gel chromatography suggested that the lectin exists mainly as the monomer and that a small fraction forms a dimer. From all the criteria examined, human placenta lectin resembles one of the chick lectins obtained from embryonic skin or adult intestine (subunit molecular weight: 14, 000). The lectin was inactivated by thiol-modifying reagents, p-chloromercuribenzoic acid and N-ethylmaleimide. Reduced and carboxymethylated lectin contained five carboxymethylated cysteines per subunit, and five free thiol groups were titrated by using 5, 5'-dithio-bis(2-nitrobenzoic acid) Preliminary sequence analysis showed the presence of a region highly homologous to the corresponding region of the chick lectin (13 identical residues out of 18 from number 70 to 87 of the chick lectin), suggesting a close evolutionary relation between these lectins and the importance of this conserved region in the function of the lectins.

1This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
T. Sasaki, J. Hirabayashi, H. Manya, K.-i. Kasai, and T. Endo
Galectin-1 induces astrocyte differentiation, which leads to production of brain-derived neurotrophic factor
Glycobiology, April 1, 2004; 14(4): 357 - 363.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.