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J. Biochem, 1988, Vol. 103, No. 2 209-211
© 1988 Japanese Biochemical Society

other

Phosphorylation of the Heavy Chain of Skeletal Muscle Myosin by Casein Kinase II: Localization of the Phosphorylation Site to the Amino Terminus1

Noriko Murakami*, Akira Kumon*, Sueo Matsumura*, Saburo Hara** and Tokuji Ikenaka**

* Department of Biochemistry, Saga Medical School Saga, Saga 840-01
** Department of Chemistry, Osaka University College of Science Toyonaka, Osaka 560

While the heavy chain of rabbit skeletal muscle myosin is not phosphorylatable by casein kinase II, it turned out to be phosphorylatable after removal of all of the light chains. The phosphorylation site for the kinase was determined to be Ser-1 and/or Ser-2 at the amino terminus.

1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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