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J. Biochem, 1988, Vol. 103, No. 2 290-296
© 1988 Japanese Biochemical Society

research-article

Molecular Cloning and the Nucleotide Sequence of cDNA for Embryonic Chicken Pepsinogen: Phylogenetic Relationship with Prochymosin1

Kensuke Hayashi, Faculty of Science*, Kiyokazu Agata**, Makoto Mochii**, Sadao Yasugi*, Goro Eguchi** and Takeo Mizuno*

* Zoological Institute,The University of Tokyo Bunkyo-ku, Tokyo 113
** Department of Developmental Biology, National Institute for Basic Biology Okazaki, Aichi 444

Embryonic chicken pepsinogen is an aspartyl proteinase that is specifically secreted during the embryonic period in the chicken proventriculus (glandular stomach). To learn the phylogeny of this pepsinogen, we isolated a cDNA clone by screening a {lambda}gt11 library of embryonic proventricular cDNAs with an antiserum to the embryonic chicken pepsinogen. We obtained a 200-base pair cDNA clone which encoded 18 amino acids that had high sequence homology with the carboxyl termini of other pepsinogens. Northern blot analysis revealed that this cDNA clone hybridized to a mRNA of 1,600 bases in the embryonic proventriculus but not to the mRNA in the adult proventriculus. The almost complete nucleotide sequence of embryonic chicken pepsinogen-cDNA was determined by sequencing longer cDNAs obtained by screening the same library with the 200-base pair cDNA and primer extension with a synthetic primer. The cDNA consisted of 1,281 nucleotides and encoded 383 amino acids for prepepsinogen. The predicted amino acid sequence was compared with the sequences of other aspartyl proteinases: pepsinogen A of human, monkey, pig, and chicken, progastricsin of monkey and rat, and bovine prochymosin. The phylogenetic tree constructed for them indicates the possibility that embryonic chicken pepsinogen diverged from prochymosin, after prochymosin and pepsinogen A had diverged from each other.

1This work was carried out under the NIBB Cooperative Research Program (85–134). It was also supported by a Grant-in-Aid (No. 61540513) from the Ministry of Education, Science and Culture of Japan.


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