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J. Biochem, 1988, Vol. 103, No. 2 313-320
© 1988 Japanese Biochemical Society

research-article

The Amino Acid Sequence of Ribonuclease U1, a Guanine-Specific Ribonuclease from the Fungus Ustilago sphaerogena1

Kenji Takahashi, Faculty of Science and Junji Hashimoto, Faculty of Science2

Department of Biophysics and Biochemistry,The University of Tokyo Bunkyo-ku, Tokyo 113

The complete amino acid sequence of ribonuclease U1 (RNase U1), a guanine-specific ribonuclease from a fungus, Ustilago sphaerogena, was determined by conventional protein sequencing, using peptide fragments obtained by several enzymatic cleavages of the performic acid-oxidized protein. The oxidized protein was first cleaved by trypsin and the resulting peptides were purified and their amino acid sequences were determined. These tryptic peptides were aligned with the aid of overlapping peptides isolated from a chymotryptic digest of the oxidized protein. The amino acid sequence thus deduced was further confirmed by isolation and analysis of peptides obtained by digestion of the oxidized protein with lysyl endopeptidase. The location of the disulfide bonds was deduced by isolation and analysis of cystine-containing peptides from a chymotryptic digest of heat-denatured RNase U1. These results showed that the protein is composed of a single polypeptide chain of 105 amino acid residues cross-linked by two disulfide bonds, having a molecular weight of 11,235, and that the NH2-terminus is blocked by a pyroglutamate residue. It has an overall homology with other guanine-specific or related ribonucleases, and shows 48% identity with RNase T1 and 38% identity with RNase U2.

1This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

2Present address: National Institute of Animal Health, Tsukuba Science City, Yatabe, Ibaraki 305.


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