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J. Biochem, 1988, Vol. 103, No. 2 332-335
© 1988 Japanese Biochemical Society

research-article

Pyridylamino Sugar Chain as an Acceptor for Galactosyltransferase1

Noriyuki Morita*, Sumihiro Hase**, Kazuhiro Ikenaka*,2, Katsuhiko Mikoshiba* and Tokuji lkenaka**

*Institute for Protein Research, Osaka University Suita, Osaka 565
**Department of Chemistry, Osaka University College of Science Toyonaka, Osaka 560

2To whom correspondence should be addressed.

Pyridylamino asialo-agalacto-biantennary sugar chain (PA-acceptor), prepared from human {alpha}1-acid glycoprotein, was incubated with bovine milk galactosyltransferase. Transfer of galactose residues to PA-acceptor was detected by HPLC analysis, and thus PA-acceptor was shown to be useful for galactosyltransferase assay. Moreover, three species of products, i.e. PA-acceptor monogalactosylated on the Man{alpha}1-3 branch of the trimannosyl core, PA-acceptor monogalactosylated on the Man{alpha}1-6 branch, and digalactosylated PA-acceptor, were separated and identified by reversed-phase HPLC, so we could simultaneously determine the branch specificity (the ratio of galactosylation on Man{alpha}1-3 branch to that on Man{alpha}1-6 branch) of the galactosyltransferase. We fractionated the bovine milk galactosyltransferase on a DEAE-5PW column and confirmed that there was a heterogeneity in this enzyme preparation. Each fraction was assayed for acceptor specificity (the ratio of the activity towards N-acetylglucosamine to that towards PA-acceptor) and branch specificity using the PA-acceptor. However, we could not detect differences in the specificities among the fractions. In addition, we found that {alpha}-lactalbumin stimulated the galactosyltransferase activity towards PA-acceptor.

1This work was supported by Special Coordination Funds from the Science and Technology Agency of the Japanese Government, a grant from the National Center for Nervous, Mental and Muscular Disorders of the Ministry of Health and Welfare and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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