J. Biochem, 1988, Vol. 103, No. 2 375-379
© 1988 Japanese Biochemical Society
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Effect of Cholestanol Feeding on Sterol Concentrations in the Serum, Liver, and Cerebellum of Mice1
* Department of Physiological Chemistry and Nutrition, The University of Tokyo Bunkyo-ku, Tokyo 113
** Department of Anatomy,The University of Tokyo Bunkyo-ku, Tokyo 113
3To whom correspondence and reprint requests should be addressed.
In order to elucidate the mechanism of xanthoma formation in cerebrotendinous xanthomatosis, mice were fed for 32 weeks with a diet rich in 5
-cholestan-3ß-ol (cholestanol) (1%, w/w). The concentrations of sterols in the serum, liver, and cerebellum were determined using high performance liquid chromatography. In the cholestanol-fed mice, the cholestanol concentrations in the serum and liver reached maxima in the first 2 to 4 weeks; the levels were about 30- to 100-fold higher than in the control diet mice. The cholestanol concentrations declined thereafter, finally to 50–60% of the maxima. Cholesterol concentrations were slightly lower in the cholestanol-fed mice throughout the experiments than in the control diet mice. On the other hand, the levels of cholestanol in the cerebellum increased almost linearly in parallel to the feeding time, and no decline was observed. These results suggest that the capacity of the liver to remove or degrade cholestanol was increased by long-term intake of this compound, whereas the cerebellum had no such feed-back regulation. Histological examinations using an electron microscope revealed the enlargement of lysosomal granules in the liver of the cholestanol-fed mice.
1This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan, and grants from the Foundation for the Promotion of Research on Medicinal Resources and the Japanese Foundation of Metabolism and Diseases.
2On leave from the Department of Nutrition and Food Science, Faculty of Science and Technology, National Fisheries University of Pusan, Pusan 607, Korea.
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