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J. Biochem, 1988, Vol. 103, No. 4 583-584
© 1988 Japanese Biochemical Society

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Rubredoxin as an Intermediary Electron Carrier for Nitrate Reduction by NAD(P)H in Clostridium perfringens

Sachiko Seki, Faculty of Pharmaceutical Sciences, Atsushi Ikeda, Faculty of Pharmaceutical Sciences and Makoto Ishimoto, Faculty of Pharmaceutical Sciences

Department of Chemical Microbiology,Hokkaido University Kita-ku, Sapporo, Hokkaido 060

The NAD(P)H-dependent nitrate reductase system in Clostridium perfringens was reconstituted with rubredoxin (Rd), nitrate reductase (NaR), and an unadsorbed fraction, on a DEAE-cellulose column, of the extract (designated as fraction A), under nitrogen gas. Ferredoxin in place of Rd was not effective as an electron carrier in this reconstituted system. NAD(P)H-dependent nitrate reducing activity was also obtained by replacing fraction A with ferredoxin-NADP+ reductase from spinach. We propose the following scheme for the electron transfer in this NAD(P)H dependent nitrate reduction system. NAD(P)H {uparrow} NAD(P)H-Rd reductase{uparrow} Rd {uparrow} NaR {uparrow} NO3–


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