Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Suminami, Y.
Right arrow Articles by Nakazawa, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suminami, Y.
Right arrow Articles by Nakazawa, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1988, Vol. 103, No. 4 611-617
© 1988 Japanese Biochemical Society

research-article

Structure and Complete Nucleotide Sequence of the Gene Encoding Chicken Cytosolic Adenylate Kinase1

Yoshinori Suminami*, Fumio Kishi**,2, Tadashi Torigoe* and Atsushi Nakazawa**,3

*Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine Ube, Yamaguchi 755
**Department of Biochemistry, Yamaguchi University School of Medicine Ube, Yamaguchi 755

3To whom correspondence should be addressed.

The gene encoding cytosolic adenylate kinase (AK1) was isolated from a chicken genomic DNA library by using its cDNA as a hybridization probe. The chicken AK1 gene spanned about 6 kilobase pairs and consisted of 7 exons. Analyses of the 5'-flanking region sequence and S1 nuclease mapping revealed that the transcription initiation site is located at 84 base pairs upstream from the ATG initiation codon. The TATA box and the putative CAT box were located 29 base pairs and 97 base pairs upstream from the transcription initiation site, respectively. A total of 7 GC boxes were found in the 5'-flanking region, the exon 1, and the intron 1. The GC boxes were surrounded by the sequences with extremely high G+C contents. When projected on the three-dimensional structure of the AK1 protein molecule, introns fell either between or near the ends of {alpha}-helices and ß-strands, and most of the coding exons encoded at least one {alpha}-helix and one ß-strand. The dot matrix plot analysis between chicken AK1 and bovine mitochondrial adenylate kinase (AK2) suggested that the AK1 gene might have evolved from the AK2 gene by deletion of one or more exon(s).

1This work was supported in part by a Grant-in-Aid for scientific research (No. 62109002) from the Ministry of Education, Science and Culture of Japan.

2Present address: Department of Pediatrics, Yamaguchi University School of Medicine, Ube, Yamaguchi 755.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. R. Elvir-Mairena, A. Jovanovic, L. A. Gomez, A. E. Alekseev, and A. Terzic
Reversal of the ATP-liganded State of ATP-sensitive K+ Channels by Adenylate Kinase Activity
J. Biol. Chem., December 13, 1996; 271(50): 31903 - 31908.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.