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J. Biochem, 1988, Vol. 103, No. 4 618-621
© 1988 Japanese Biochemical Society


research-article

Structure of 6-Deoxytalose-Containing Polysaccharide from the Cell Wall of Bifidobacterium adolescentis

Masato Nagaoka, Masami Muto, Teruo Yokokura and Masahiko Mutai

Yakult Central Institute for Microbiological Research Kunitachi, Tokyo 186

The isolation and analysis of the cell wall and the polysaccharide-glycopeptide complexes of Bifidobacterium adolescentis YIT4011 are presented. Polysaccharide-glycopeptide complexes, PS-GP1 and PS-GP2, were solubilized from the cell wall by treatment with N-acetylmuramidase. PS-GP1 and PS-GP2 were found to be composed of glucose, 6-deoxytalose and a small amount of glycopeptide. The products of Smith degradation of the PS-GPs had no glucose-containing fraction, but were composed of 1,2/1,3-linked 6-deoxytalose. Furthermore, a second Smith degradation of this fraction yielded trisaccharide-glyceraldehyde. These results and methylation analysis led to the conclusion that PS-GP1 or 2 has a repeating unit of ->3) 6dTal(ß1->3)6dTal(ß1->3)6dTal(ß12)-6dTal({alpha}l->2)6dTal({alpha}l->2)6dTal({alpha}l-, and that glucose residues are linked to position C-3 of the 2-O-substituted 6-deoxytalose residues.


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