J. Biochem, 1988, Vol. 103, No. 4 618-621
© 1988 Japanese Biochemical Society
research-article |
Structure of 6-Deoxytalose-Containing Polysaccharide from the Cell Wall of Bifidobacterium adolescentis
Yakult Central Institute for Microbiological Research Kunitachi, Tokyo 186
The isolation and analysis of the cell wall and the polysaccharide-glycopeptide complexes of Bifidobacterium adolescentis YIT4011 are presented. Polysaccharide-glycopeptide complexes, PS-GP1 and PS-GP2, were solubilized from the cell wall by treatment with N-acetylmuramidase. PS-GP1 and PS-GP2 were found to be composed of glucose, 6-deoxytalose and a small amount of glycopeptide. The products of Smith degradation of the PS-GPs had no glucose-containing fraction, but were composed of 1,2/1,3-linked 6-deoxytalose. Furthermore, a second Smith degradation of this fraction yielded trisaccharide-glyceraldehyde. These results and methylation analysis led to the conclusion that PS-GP1 or 2 has a repeating unit of
3) 6dTal(ß1
3)6dTal(ß1
3)6dTal(ß12)-6dTal(
l
2)6dTal(
l
2)6dTal(
l-, and that glucose residues are linked to position C-3 of the 2-O-substituted 6-deoxytalose residues.