Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Muto, N.
Right arrow Articles by Yonezawa, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muto, N.
Right arrow Articles by Yonezawa, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1988, Vol. 103, No. 4 629-632
© 1988 Japanese Biochemical Society

research-article

Characteristic Distribution of Cathepsin E Which Immunologically Cross-Reacts with the 86-kDa Acid Proteinase from Rat Gastric Mucosa

Norio Muto, Faculty of Pharmaceutical Sciences*,1, Makoto Yamamoto, Faculty of Pharmaceutical Sciences*, Satoru Tani, Faculty of Pharmaceutical Sciences* and Satoshi Yonezawa, Faculty of Science**

*Josai University Sakado, Saitama 350-02
**Hokkaido University Kita-ku, Sapporo, Hokkaido 060

1To whom correspondence should be addressed at: Faculty of Pharmaceutical Science, Okayama Univerisity, Okayama, Okayama 700.

The antiserum raised against the high-molecular-weight acid proteinase from rat gastric mucosa, termed 86-kDa acid proteinase, has been shown to recognize rat cathepsin E, but not cathepsin D (Muto, N. et al. (1987) J. Biochem. 101, 1069–1075). Using this specific antiserum, characteristic distribution of cathepsin E in rats was demonstrated. The enzyme was detected in a limited number of tissues, such as stomach, thymus, spleen, bladder, and erythrocyte membranes. Among them, the highest activity was observed in the stomach. In contrast, cathepsin D immunoreactive with the antiserum specific to rat gastric cathepsin D was demonstrated in all the tissues examined. Cathepsin E-type enzymes partially purified from these five tissues were precipitated in the same manner by the specific antiserum, and they had the same molecular weight, electrophoretic mobility, and resistance against denaturation by 4 M urea. These results indicate that they could be exactly classified as cathepsin E. This type of enzyme was also detectable in mice and guinea pigs, but they showed relatively weak immunoreactivities with the antiserum. Thus, it is concluded that the distribution of cathepsin E is intrinsically different from ordinary cathepsin D, suggesting that it has a different physiological role from cathepsin D.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
F. Henningsson, K. Yamamoto, P. Saftig, T. Reinheckel, C. Peters, S. D. Knight, and G. Pejler
A role for cathepsin E in the processing of mast-cell carboxypeptidase A
J. Cell Sci., May 1, 2005; 118(9): 2035 - 2042.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. Ikuzawa, S. Yasumasu, T. Inokuchi, K.-i. Kobayashi, K. Nomura, and I. Iuchi
Differential Expression of Two Cathepsin Es during Metamorphosis-Associated Remodeling of the Larval to Adult Type Epithelium in Xenopus Stomach
J. Biochem., September 1, 2003; 134(3): 385 - 394.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nishioku, K. Hashimoto, K. Yamashita, S.-Y. Liou, Y. Kagamiishi, H. Maegawa, N. Katsube, C. Peters, K. von Figura, P. Saftig, et al.
Involvement of Cathepsin E in Exogenous Antigen Processing in Primary Cultured Murine Microglia
J. Biol. Chem., February 8, 2002; 277(7): 4816 - 4822.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.