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J. Biochem, 1988, Vol. 103, No. 4 641-643
© 1988 Japanese Biochemical Society

other

A Rapid Purification with High Recovery of Factor XII (Hageman Factor) on Immunoaffinity Column: Application to an Abnormal Clotting Factor XII (Factor XIITORONTO)1

Isao Takahashi and Hidehiko Saito2

First Department of Internal Medicine, Nagoya University School of Medicine Showa-ku, Nagoya, Aichi 466

2 To whom correspondence should be addressed.

A rapid purification procedure with high recovery of blood coagulation factor XII (Hageman factor, HF) was established. Homogeneous HF was isolated in 6 days on a monoclonal antibody-immunoaffinity column chromatography followed by gel filtration. Approximately 4,300-fold purification of HF was attained with 31% yield on average (n= 4). Using this method, an abnormal HF was purified from plasma of a patient with cross-reacting material (CRM)-positive Hageman trait (factor XIITORONTO). The abnormal HF was found to be a single chain polypeptide with the same molecular weight (80,000) as the normal HF. Both abnormal and normal HF had similar amino acid compositions.

1 This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture and Grants from the Ministry of Health and Welfare of Japan.


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