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J. Biochem, 1988, Vol. 103, No. 5 787-791
© 1988 Japanese Biochemical Society

research-article

Thrombin-Induced Membrane Depolarization of Platelets and Its Inhibition by Cetiedil1

Atsumi Yamaguchi*, Hiroshi Azuma**, Satomi Sekizaki**, Hidenori Suzuki*, Kenjiro Tanoue* and Hiroh Yamazaki*

* Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113
** Department of Medicinal Chemistry, Institute for Medical and Dental Engineering, Tokyo Medical and Dental University Chiyoda-ku, Tokyo 101

When 50 µM cetiedil alone was added to a platelet suspension, increase in Na+ content, decrease in K+ content, and depolarization of platelet membrane were observed without change in the intracellular concentration of free Ca2+ ([Ca2+]1) or in the morphology of platelets. The cetiedil-induced depolarization was attenuated by the reduction of extracellular sodium concentration, while sodium transport inhibitors such as procaine and tetrodotoxin failed to modify the depolarization. On the other hand, thrombin caused such changes in platelets as increases in Na+ content, 22Na space and [Ca2+]1, decrease in K+ content, and membrane depolarization. All these changes caused by thrombin were inhibited by cetiedil. It is suggested that cetiedil brought the increased ion transport and subsequent partial depolarization, which might lead to modification of the reaction of platelet membrane induced by thrombin.

1This work was supported by Special Coordination Funds for Promoting Science and Technology Agency of Japan, and by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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