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J. Biochem, 1988, Vol. 103, No. 5 884-888
© 1988 Japanese Biochemical Society

research-article

Monoclonal Antibodies against Discoidin I and Discoidin II of the Cellular Slime Mold, Dictyostelium discoideum1

Masashi Fukuzawa, Faculty of Science and Hiroshi Ochiai, Faculty of Science2

Department of Botany,Hokkaido University Kita-ku, Sapporo, Hokkaido 060

2 To whom correspondence should be addressed.

The preparation and properties of monoclonal antibodies against carbohydrate-binding proteins (discoidin I and discoidin II) in the cellular slime mold, Dictyostelium discoideum are described. Monoclonal antibody (mAb) ndI, II-1 bound both discoidins I and II specifically. mAb nl-1 and mAb dl-1 bound only discoidin I but their binding specificities were different: nl-1 recognized the native form and dl-1 the denatured form. mAb dll-1 bound only denatured discoidin II. In preliminary work mAbs dII-1 and nl-1 were found to be useful for localizing discoidins I and II immunohistochemically.

1This study was supported in part by Grants-in-Aid for Scientific Research and for Co-operative Research from the Ministry of Education, Science and Culture of Japan.


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