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J. Biochem, 1988, Vol. 103, No. 6 954-961
© 1988 Japanese Biochemical Society

research-article

Construction of a Human Cytochrome c Gene and Its Functional Expression in Saccharomyces cerevisiae

Yoshikazu Tanaka, Faculty of Science*, Toshihiko Ashikari*, Yuji Shibano*, Teruo Amachi*, Hajime Yoshizumi* and Hiroshi Matsubara**

* Institute for Fundamental Research, Research Center, Suntory, Ltd. Shimamoto-chou, Mishima-gun, Osaka 618
** Department of Biology,Osaka University Toyonaka, Osaka 530

The nucleotide sequences of a partial cDNA and three pseudogenes of human cytochrome c were determined. The complete nucleotide sequences which encode human cytochrome c were constructed on the basis of one of the pseudogenes by in vitro mutagenesis. The constructed human cytochrome c was functionally expressed in Saccharomyces cerevisiae. The recombinant human cytochrome c was purified and characterized.


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