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J. Biochem, 1988, Vol. 103, No. 6 998-1003
© 1988 Japanese Biochemical Society

research-article

Glycolipids of Metastatic Tissue in Liver from Colon Cancer: Appearance of Sialylated Lex and Lex Lipids1

Takao Taki*,2, Miki Takamatsu*, Akio Myoga**, Koichi Tanaka***, Susumu Ando*** and Makoto Matsumoto*

* Department of Biochemistry, School of Pharmaceutical Science, University of Shizuoka Shizuoka, Shizuoka 422
** Institute of Dainabot Co., Ltd. Matsudo, Chiba 271
*** Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology Itabashi-ku, Tokyo 173

2To whom correspondence should be addressed.

Glycolipids of metastatic tissue in liver from colon cancer were separated by HPLC and their structures were analyzed. Neutral glycolipid was composed of five major components. Four of them showed similar mobilities to GlcCer, LacCer, Gb3Cer, and nLc4Cer on TLC. The structure of the fifth major component was analyzed by exo-glycosidase treatments, 400 MHz proton NMR spectrometry, gas chromatography-mass spectrometry of the methylated sugars, and immunostaining on a TLC-plate, and concluded to be


Formula

Ganglioside was composed of two major components. One of them was identical with GM3. The structure of the second ganglioside was analyzed by exo-glycosidase treatment, immunostaining on a TLC-plate, and gas chromatography-mass spectrometry of methylated sugars, and concluded to be


Formula

These Lex and sialyl-Lex structure-containing lipids accounted for about 12% of the neutral glycolipid fraction and 42% of the ganglioside fraction, respectively. From these results, it appears that the biosynthetic pathway for Lex structure-containing lipids is activated in colon cancer.

1This work was supported in part by a Grant-in Aid from the Minitry of Education, Science and Culture of Japan.


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