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J. Biochem, 1988, Vol. 104, No. 1 12-13
© 1988 Japanese Biochemical Society

other

Crystallization of and Preliminary Crystallographic Data for Bacillus stearothermophilus Cyclodextrin Glucanotransferase

Michio Kubota**, Bunzo Mikami*, Yoshio Tsujisaka** and Yuhei Morita*,1

*Research Institute for Food Science, Kyoto University Uji, Kyoto 611
**Research Laboratory Hayashibara Co., Ltd., Okayama, Okayama 700

1 To whom correspondence should be addressed

Cyclodextrin glucanotransferase from Bacillus stearothermophilus TC-91 has been crystallized from an ammonium sulfate solution by the dialysis equilibrium method. The crystals belong to the orthorhombic system, space group P212121 with cell dimensions of a = 125.5 Å, b = 88.1 Å, and c = 81.5 Å. The crystals appear to be suitable for X-ray structure analysis, diffracting to at least 2.1 Å and being resistant to radiation damage.


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