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J. Biochem, 1988, Vol. 104, No. 1 18-21
© 1988 Japanese Biochemical Society

research-article

Simple Assay for Sialyltransferase Activity with a New Fluorogenic Substrate1

Takayuki Sato, Kaoru Omichi2 and Tokuji Ikenaka

Department of Chemistry, Osaka University College of Science Toyonaka, Osaka 560

2 To whom correspondence should be addressed

A new fluorogenic acceptor for sialyltransferase, 2- [(2-pyridyl)amino]ethyl O-ß-D-galactopyranosyl-(1->4)-ß-D-glucopyranocide, was prepared from lactose as a starting material. Sialyltransferase activity was assayed by incubation of the enzyme with the acceptor and CMP-N-acetylneuraminic acid, separation of the fluorogenic sialylated product from the enzymatic reaction mixture by HPLC, and measurement of the product. Compared to assays so far reported that use radioactive substrates, this assay is simple and rapid. This method was used to assay sialyltransferase activity in human serum.

1 This study was reported in part at the 60th Annual Meeting of the Japanese Biochemical Society.


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