J. Biochem, 1988, Vol. 104, No. 1 30-34
© 1988 Japanese Biochemical Society
research-article |
High-Level Expression of Human BSF-2/IL-6 cDNA in Escherichia coli Using a New Type of Expression-Preparation System
*Central Research Laboratories Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki, Kanagawa 210;
**Institute for Molecular and Cellular Biology, Osaka University Suita, Osaka 565
BSF-2 (B cell stimulatory factor-2/IL-6) is a member of the lymphokine family and responsible for B celldifferentiation. Expression plasmids of human BSF-2 cDNA were constructed using a trp promotor/operator and a trpA terminator. In an extract of Escherichia coli HB101 holding "direct" expression plasmid pBSF-2D, activity of BSF-2 was detected, but overproduction wasnot observed. A "fused" expression system was therefore developed to prepare the recombinant protein. In this system, cDNA was expressed as a fused protein with human IL-2 N-terminal peptide. In the case of thefused BSF-2 expression plasmid, pBSF-2F, inclusion bodies were observed and overproduction of the protein occurred. As this fused protein had a Phe-Arg-Ala sequence at the junction of hIL-2 and BSF-2, it was possible to process mature BSF-2 from the fused BSF-2 by treatment with kallikrein and aminopeptidase P. From 1 liter of E. coli culture, 45 mg of mature BSF-2 was purified; it had a relative biological activity equal to that of naturalBSF-2 purified from T cells.