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J. Biochem, 1988, Vol. 104, No. 1 40-43
© 1988 Japanese Biochemical Society

research-article

Synthesis of Sex-Specific Forms of Cytochrome P-450 in Rat Liver Is Transiently Suppressed by Hepatic Monooxygenase Inducers

Yoshikazu Emi and Tsuneo Omura

Department of Molecular Biology, Graduate School of Medical Science, Kyushu University Higashi-ku, Fukuoka, Fukuoka 812

The effects of phenobarbital (PB), 3-methyicholanthrene (MC), and {alpha}-naphthoflavone ({alpha}-NF) on the synthesis of drug-inducible forms of cytochrome P-450, P-450(PB-1), and P-450(MC-1), and sex-specific forms of cytochrome P-450, P-450(M-1), and P-450(F in male and female rats were studied. Whereas P-450(PB-1) and P-450(MC-1) in liver microsomes were markedly induced in both sexes by treatment with PB and MC, respectively, the contents of P-450(M-1) and P-450(F-1) were significantly decreased by the treatments. {alpha}-NF, which is not a P-450 inducer, did not change the contents of sex-specific forms of cytochrome P-450. The translatable mRNAs of the P-450s were also determined by using an in vitro translation system. The mRNAs coding for P-450(PB-1) and P-450(MC-1) were increased by drug administrations. On the other hand, the mRNAs coding for P-450(M-1) and P-450(F-1) were transiently decreased by the drugs, and then returned to the normal levels. The time courses of the induction of the drug-inducible P-450s and the repression of the sex-specific P-450s showed no close correlation. {alpha}-NF had no effect on the synthesis of P-450(M-1) and P-450(F-1). We also found that the synthesis of P-450(M-1) in the livers of untreated rats showed no diurnal variations.


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