J. Biochem, 1988, Vol. 104, No. 1 81-86
© 1988 Japanese Biochemical Society
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Changes in Aerobic and Anaerobic ATP-Synthesizing Activities in Hypoxic Mouse Brain1
*Department of Physiological Chemistry, Osaka University Medical School Kita-ku, Osaka, Osaka 530
**First Department of Internal Medicine, Osaka University Medical School Kita-ku, Osaka, Osaka 530
1 To whom correspondence should be addressed
The changes in cerebral metabolism in mice in severe hypoxia were investigated by analyses of changes in the levels of energy metabolites and near-infrared spectro photometric assessment of the states of hemoglobin and cytochrome oxidase. Under 4.4% 02, the contribution of anaerobic ATP production was at most about 20% of the demand. However, the cerebral ATP level was kept at the control level until about 1 mm before death. Pentobarbital anesthesia, which reduced the cerebral rate of metabolism, prolonged the survival time, although anaerobic ATP production still did not support ATP demand. Under these conditions, deoxygenation of hemoglobin and reduction of cytochrome oxidase proceeded rapidly within 1 mm. Hemoglobin reached the maximum state of deoxygenation in the middle phase of hypoxia, with no further change until death. However, cytochrome oxidase was reduced slowly with one phase of partial reoxidation due to increase of cere bral blood volume, and reached the completely reduced state at death. From these results it was concluded that the aerobic ATP synthesis, which supplied more than 80% of the cerebral demand, decreased gradually because of limitation of oxygen supply, and that the failure of oxidative phosphorylation to meet demand triggered the decrease in the cellular ATP level that led to death.
This work was supported in part by Grants.in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
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