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J. Biochem, 1988, Vol. 104, No. 1 9-11
© 1988 Japanese Biochemical Society

other

Separation of Akazara Scallop and Rabbit Troponin Components by a Single-Step Chromatography on CM-Toyopearl

Takao Ojima and Kiyoyoshi Nishita

Department of Chemistry, Faculty of Fisheries, Hokkaido University Hakodate, Hokkaido 041

Three components of Akazara scallop (Chlamys nipponensis akazara) troponin were well separated from each other by a single-step chromatography on CM-Toyopearl, although they were hardly separated on DEAE-Sephadex A-25. Moreover, by means of this CM-chromatography, the troponin components of rabbit were also readily separated with high purities and in high yields. The components thus separated were readily reconstituted and the Ca2+regulatory function was fully recovered.


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