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J. Biochem, 1988, Vol. 104, No. 3 337-343
© 1988 Japanese Biochemical Society

research-article

Biased Expression of Variable Region Gene Families of the Immunoglobulin Heavy Chain in Autoimmune-Prone Mice1

Yoshio Yaoita*, Masazumi Takahashi*, Chihiro Azuma*, Yoshiyuki Kanai** and Tasuku Honjo*,2

*Department of Medical Chemistry, Kyoto University Faculty of Medicine Sakyo-ku, Kyoto, Kyoto 606
**Department of Molecular Oncology, Institute of Medical Science, The University of Tokyo Minato.ku, Tokyo 108

2To whom correspondence should be addressed.

We have examined usage of variable region gene families of the immunoglobulin heavy chain (VH gene family) in spleens of MRL/MpJ-lpr/lpr (MRL/lpr), (NZB×NZW)F1, and BXSB mice by Northern analysis using various VH probes, including the VHPAR gene which we cloned and identified as a gene encoding the heavy-chain variable region of antipoly(ADP-ribose) antibody. The amount of VHS107 family mRNA was almost constant for the same amount of splenic crude RNA in autoimmune-prone and normal mice, while concentrations of other family mRNAs were elevated in autoimmune-prone mice. For example, per splenic RNA the VHPAR family was expressed in MRL/lpr mice 10 times more than in their normal counterpart, MRL/MpJ-+/+ (MRL/+) mice. These results indicate the bias of V gene usage in autoimmune-prone mice. Expression of the VHS107 family was depressed from an early life stage of MRL/lpr and male BXSB mice. Furthermore, the expression of IL-4 and IL-5 were quantitatively compared, as B cell differentiation factor was thought to be produced by abnormally proliferative T cells in lymph nodes of MRL/lpr mice. We could not, however, observe overproduction of IL-4 and IL-5 mRNA in the lymph nodes.

1This investigation was supported by a Grant-in-Aid from Ministry of Health and Welfare of Japan.


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