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J. Biochem, 1988, Vol. 104, No. 3 344-348
© 1988 Japanese Biochemical Society

research-article

Cation Channels from Ciliary Membrane of Tetrahymena Reconstituted into Planar Lipid Bilayer. Comparison between the Channels from the Wild T. Thermophila and from Its Mutant Which Does Not Show Ciliary Reversal1

Chie Fujiwara*, Kazunori Anzai*, Yutaka Kirino*,2, Seiji Nagao**, Yoshinori Nozawa** and Mihoko Takahashi***

*Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku, Fukuoka, Fukuoka 812
**Gifu University School of Medicine Gifu, Gifu 500
***Institute of Biological Sciences, University of Tsukuba Tsukuba, Ibaraki 305

2To whom correspondence should be addressed.

Cation channels in ciliary membrane vesicles from wild type Tetrahymena thermophila and from its mutant which does not show ciliary reversal or avoiding reaction were reconstituted into a planar lipid bilayer. Since the mutant does not produce the regenerative Ca2+ action potential, the mutation was expected to have occurred at the Ca-channel in the ciliary membrane. In the sample from the mutant, the channel most frequently observed was selective for cations over anions. The single channel conductance shows MichaelisMenten type dependency on the cation concentration. The maximum conductance and dissociation constants (in parenthesis) for K+, Mg2+ Ca2+ and Ba2+ were 371 PS (23.3 mM), 17 pS (0.49 mM), 18 pS (0.52 mM), and 25 pS (0.82 mM), respectively, when the anion was gluconate. The properties of the corresponding channel from the wild type are similar to those from the mutant. No essential difference was detected, which indicates that the predominant channel is not the putative Ca channel responsible for the avoiding reaction of Tetrahymena. Some other channels than the predominant channel were also observed.

1This study was supported in part by Grants.in-Aid for Special Project Research (No. 61107006 and 61215024) and a Grant-in.Aid for Co-operative Research (No. 62304061) from the Ministry of Education, Science and Culture of Japan.


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