Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Oyama, F.
Right arrow Articles by Uchida, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oyama, F.
Right arrow Articles by Uchida, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1988, Vol. 104, No. 3 403-408
© 1988 Japanese Biochemical Society

research-article

A Synthetic, Partial Pre-mRNA for Ovalbumin Primes Its Own Complementary DNA with Reverse Transcriptase

Fumitaka Oyama1, Rie Kikuchi and Tsuneko Uchida2

Mitsubishi Kasei Institute of Life Sciences Machida, Tokyo 194

1To whom correspondence should be addressed

Synthetic, partial pre-mRNA for ovalbumin was found to prime its own cDNA with reverse transcriptase. Initiation of the cDNA occurred 36 bases upstream of the 3'-end of the RNA, probably as a result of intramolecular base pairing at this end. Inhibition of self-priming occurs following ligation of pCp or poly-adenylation at the 3'-terminus of the synthetic RNA. The secondary structure of 3'-end region of the template RNA strongly affected the ability of self-priming.

1Present address: Institute for Comprehensive Medical Science, Fujita.Gakuen Health University, Toyoake, Aichi 470-11.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.