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J. Biochem, 1988, Vol. 104, No. 3 461-465
© 1988 Japanese Biochemical Society

research-article

Calcium Release from Isolated Sarcoplasmic Reticulum Due to 4,4'-Dithiodipyridine1

Satoshi Nagura, Takashi Kawasaki, Takahisa Taguchi and Michiki Kasai2

Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University Toyonaka, Osaka 560

2To whom correspondence should be addressed

The effects of SH reagents on Ca2+ release from sarcoplasmic reticulum (SR) vesicles were examined by the tracer method using 45Ca2+. Among the various SH reagents tested, 4,4'-dithiodipyridine (PDS) was found to induce Ca2+ release most specifically from the heavy fraction of SR vesicles. Further, the following results were obtained. (i) PDS bound covalently to proteins in the SR membrane and induced Ca2+ release. (ii) The Ca2+ release was further enhanced by ATP and caffeine, but inhibited by procaine, ruthenium red and various divalent cations. (iii) PDS enhanced the Ca2+ release in the whole range of Ca2+ concentrations tested. (iv) Choline permeability was also enhanced by PDS. Further, the electrical conductance of the Ca2+-induced Ca2+ release channels was studied by incorporating them into lipid bilayers and it was found that PDS increased the probability of opening of the channels. These results suggest that PDS binds to certain SH groups of the Ca2+ induced Ca2+ release channels in the SR membrane and thus induces Ca2+ release.

1This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas of "Bioenergetics" to M.K. from the Ministry of Education, Science and Culture of Japan.


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