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J. Biochem, 1988, Vol. 104, No. 5 683-686
© 1988 Japanese Biochemical Society

other

Expression of Two Kinds of Cytochrome P-450(11ß) mRNA in Bovine Adrenal Cortex

Shirou Kirita*, Ken-ichirou Morohashi**, Toshihide Hashimoto**, Hidefumi Yoshioka***, Yoshiaki Fujii-Kuriyama**** and Tsuneo Omura**

*Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka, Fukuoka 812
**Department of Molecular Biology, Graduate School of Medical Science, Kyushu University Higashi-ku, Fukuoka, Fukuoka 812
***Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park North Carolina 27709, U.S.A.
****Department of Chemistry, Faculty of Science, Tohoku University Sendai, Miyagi 980

Using pcP-450(11ß)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559–568) as the probe, a different cDNA clone, pcP-450(11ß)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11ß)-3 was highly homologous but not identical with that of pcP-450(11ß)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11ß)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11ß) mRNA were expressed in individual animals and that a least two kinds of P-450(11ß) genes exist in the bovine genome.


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