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J. Biochem, 1988, Vol. 104, No. 5 700-705
© 1988 Japanese Biochemical Society

research-article

Complete Amino Acid Sequence of Ferredoxin from Peridinium bipes (Dinophyceae)

Aritsune Uchida*, Shinya Ebata*, Keishirou Wada**,1, Hiroshi Matsubara** and Yuzaburo Ishida*

*Laboratory of Microbiology, Department of Fisheries, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto, Kyoto 606
**Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka 560

The amino acid sequence of the major ferredoxin component isolated from a dinoflagellate, Peridinium bipes, was completely determined. Staphylococcus aureus V8 proteolytic, tryptic and chymotryptic peptides of Cm-ferredoxin were prepared and sequenced. The sequence was Phe-Lys-Val-Thr-Leu-Asp-Thr-Pro-Asp-Gly-Lys-Lys-Ser-Phe-Glu-Cys-Pro-Gly-Asp-Ser-Tyr-Ile-Leu-Asp-Lys-Ala-Glu-Glu-Glu-Gly-Leu-Glu-Leu-Pro-Tyr-Ser-Cys-Arg-Ala-Gly-Ser-Cys-Ser-Ser-Cys-Ala-Gly-Lys-Val-Leu-Thr-Gly-Ser-Ile-Asp-Gln-Ser-Asp-Gln-Ala-Phe-Leu-Asp-Asp-Asp-Gln-Gly-Gly-Asp-Gly-Tyr-Cys-Leu-Thr-Cys-Val-Thr-Tyr-Pro-Thr-Ser-Asp-Val-Thr-Ile-Lys-Thr-His-Cys-Glu-Ser-Glu-Leu. It was composed of 93 amino acid residues with 7 cysteine residues, the highest number found among the chioroplast-type ferredoxins so far sequenced. A cysteine residue was found for the first time at the 89th position in a chioroplast-type ferredoxin. Calculation of the numbers of amino acid differences among chloroplast-type ferredoxins indicates that the Peridinium ferredoxin is far divergent not only from higher plant ferredoxins but also from blue-green algal ferredoxins.

1 Present address: Department of Biology, Faculty of Science, Kanazawa University, Kanazawa, Kanazawa 920.


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