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J. Biochem, 1988, Vol. 104, No. 5 738-741
© 1988 Japanese Biochemical Society

research-article

Poly-N-Acetyllactosamines Synthesized by Cultured Ehrlich Carcinoma Cells: Application of Endo-ß-Galactosidase C for Analysis of the Terminal Structure1

Yuko Kamada*, Hisako Muramatsu*, Makoto Kawata**, Hiroyoshi Takamizawa** and Takashi Muramatsu*,2

*Department of Biochemistry, Faculty of Medicine, Kagoshima University Kagoshima, Kagoshima 890
**Department of Obstetrics and Gynecology, Chiba University School of Medicine Chiba, Chiba 280

2 To whom correspondence should be addressed

Poly-N-acetyllactosamines were prepared from Ehrlich carcinoma cells cultured in the presence of [14C] galactose. Methylation analysis indicated that 31% of the galactose was in the non-reducing end. Of it, 77% was cleaved by {alpha}-galactosidase, and 56% was released as a disaccharide by endo-ß-galactosidase C. Methylation analysis confirmed that the released disaccharide was mostly Gal {alpha}1->3Gal. Therefore, Gal {alpha}1->3Gal structure, not Gal{alpha}1->3(Gal{alpha}1->6)Gal structure, was the major {alpha}-galactosyl structure in the poly-N-acetyllactosamines synthesized. Furthermore, {alpha}-galactosidase digestion did not change the content of disubstituted galactosyl residues. Thus, Gal{alpha}1->3(Gal{alpha}1->6)Gal structure, which was suggested to be the sole non-reducing terminal structure of poly-N-acetyllactosamines of Ehrlich carcinoma cells, was not detected in significant amounts under the present experimental conditions.

1 This study was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan. All sugars mentioned in this paper have a D-configuration, except for fucose, which has an L-configuration.


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