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J. Biochem, 1988, Vol. 104, No. 5 855-857
© 1988 Japanese Biochemical Society

research-article

Relation between ESR-Detectable Cu(II) and Superoxide Dismutase Activity

Jun-ichi Kajihara*, Mitsuo Enomoto*, Kazuo Katoh*, Keiichi Mitsuta** and Masahiro Kohno**

*Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd. Kita-ku, Tokyo 115
**ESR Group Application Lab., Technical and Engineering Division, JEOL, Ltd. Akishima, Tokyo 196

The relation between ESR-detectable Cu(II) and Cu,Zn-superoxide dismutase activity was examined. The Cu(II) spin numbers per one unit of SOD were 6.26×1012 (±0.51×1012) spins in several preparations of recombinant human Cu,Zn-SOD, native placental, and eryth rocyte SOD. Measurement could be performed over a wide range of pH (4.0-10.0), preferably at temperatures below –40°C. The data obtained by this method correlated well to the results obtained by the method of Fridovich et al. using the xanthine-xanthine oxidase system (correlation coefficient 0.995). The specific activity of SOD was proportional to the Cu(II) content measured by ESR, but not to the total Cu content measured by atomic absorption. This indicates that it is important to measure the Cu(II) content for determining Cu,Zn-SOD activity.


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