J. Biochem, 1989, Vol. 106, No. 1 1-4
© 1989 Japanese Biochemical Society
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Identification and Characterization of an L1 Family Sequence with a Very Long Open Reading Frame in the Third Intron of the Human Thy midy late Synthase Gene1
*School of Food and Nutritional Sciences, University of Shizuoka, Yada, Shizuoka, Shizuoka 422
**Institut National de la Recherche Scientifique, Universite du Québec, Pointe- Claire, Quebec H9R 1G6, Canada
***Laboratory of Mutagenesis, National Institute of Genetics, Mishima, Shizuoka 411
3To whom correspondence should be addressed
A long L1 repetitive sequence (3.6 kilobase pairs) was found in the third intron of the human thymidylate synthase gene. This Ll family sequence is unique in that it possesses the longest open reading frame (1.7 kilobase pairs) of all Ll family members identified in sequences associated with specific genes that have been cloned thus far. Furthermore, the amino acid sequence deduced from the open reading frame of the Ll sequence was found to be highly homologous (90%) to that encoded by a known human teratocarcinoma Ll RNA species, and to contain several blocks of sequences homologous to ones in RNA-dependent DNA polymerases of various origins.
2Present address: Institute of Applied Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo 113.
1This work was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan, and by a grant from the Fugaku Trust for Medical Research.