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J. Biochem, 1989, Vol. 106, No. 1 115-118
© 1989 Japanese Biochemical Society

research-article

Z-Protein, a Component of the Skeletal Muscle Z-Line, Is Located at the Apical Tips of Microvilli of Chicken Intestinal Epithelial Cells1

Kazuyo Ohashi and Koscak Maruyama

Department of Biology, Faculty of Science, Chiba University Chiba, Chiba 260

Antiserum against Z-protein, which is one of the components of myofibrillar Z-lines, strongly stained the epithelial cells of chicken small intestine at the apical tips of the microvilli and the junctional complexes. Immunoblot tests with anti-Z-protein antiserum showed that there is a peptide of the same antigenicity as myofibrillar Z-protein in the components of the epithelial cells of chicken small intestine. Hence it seems that the apical tip of the microvillus contains a constituent corresponding to the Z-protein of myofibrillar Z-line.

1This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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