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J. Biochem, 1989, Vol. 106, No. 1 34-37
© 1989 Japanese Biochemical Society

research-article

Altered Ganglioside Expression in ras-Oncogene-Transformed Cells1

Yasuo Suzuki*,2, Kazuya Hidari*, Makoto Matsumoto*, Masa-aki Ikeda** and Nobuo Tsuchida**

*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science Shizuoka, Shizuoka 422
**Department of Microbiology, Faculty of Dentistry, Tokyo Medical and Dental University Bunkyo-ku, Tokyo 113

2To whom correspondence should be addressed.

Alteration of ganglioside composition in mouse BALB/3T3 cells transformed either by DNA transfection with viral K-, H-, or cellular H-ras oncogene, or by infection with the K-ras oncogen-carrying murine sarcoma virus (Ki-KSV) was studied using a highly sensitive thin-layer chromatography/enzyme immunostaining method. Marked common decreases in the content of GD3 ganglioside and the increase of its metabolic precursor GM3 were bound in BALB/3T3 cell lines transformed by either K- or H- ras oncogenes. Moreover, a common decrease or loss in the contents of "A" series ganglio-tetraose gangliosides such as GM1a and GD1a was also found in all transformed cell lines, indicating that the alteration of cellular glycosphingolipids by ras oncogenes apparently does not depend on the type of ras-concogenes (K- and H-ras).

1This work was supported in part by a Grant-in-Aid (63480493) from the Ministry of Education, Science and Culture of Japan.


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