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J. Biochem, 1989, Vol. 106, No. 1 55-59
© 1989 Japanese Biochemical Society

research-article

Amino Acid Sequence of Porcine Cardiac Muscle Troponin C1

Tomoyoshi Kobayashi*, Takashi Takagi*,2, Kazuhiko Konishi*, Sachio Morimoto** and Iwao Ohtsuki**

*Biological Institute, Faculty of Science, Tohoku University Sendai, Miyagi 980
**Department of Pharmacology, Faculty of Medicine, Kyushu University, Higashi-ku Fukuoka, Fukuoka 812

2To whom correspondence and reprint requests should be addressed.

Troponin C is the Ca2+-receptive protein located on the thin filament of striated and cardiac muscle. We have determined the amino acid sequence of troponin C obtained from porcine cardiac muscle by sequencing and aligning the lysyl endopeptidase and Staphylococcus aureus V-8 protease peptides. It was composed of 161 amino acid residues with a blocked N-terminus. The sequence of porcine cardiac troponin C was identical with that of bovine cardiac troponin C.

1This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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