Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Tsugita, A.
Right arrow Articles by Shikama, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsugita, A.
Right arrow Articles by Shikama, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1989, Vol. 106, No. 1 60-65
© 1989 Japanese Biochemical Society

research-article

Sensitization of Edman Amino Acid Derivatives Using the Fluorescent Reagent, 4-Aminofluorescein1

Akira Tsugita*, Masaharu Kamo*, Carl S. Jone* and Noriko Shikama**

*Research Institute for Biosciences, Science University of Tokyo Noda, Chiba 278
**Department of Applied Biological Science, Science University of Tokyo Noda, Chiba 278

The ability to analyze amino acid derivatives at the femtomole level is one of the most interesting challenges in the field of protein microsequencing. 2-Anilino-5-thiazolinone amino acids, obtained by Edman degradation, were quantitatively derivatized with fluorescent primary amines. The most fluorescent reagent tested was 4-aminofluorescein. The amino acid derivatives sensitized with this reagent were separated using reversed-phase high-performance liquid chromatography and identified at the 100 attomole level. Incorporation of this method into the operation of a conventional automated sequencer is also described.

1This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan. One of the authors (CSJ) was a recipient of a scholarship from the Ciba-Geigy Foundation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.