J. Biochem, 1989, Vol. 106, No. 1 8-10
© 1989 Japanese Biochemical Society
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Aluminum Enhances the Stimulatory Effect of NaF on Prostaglandin E2 Synthesis in a Clonal Osteoblast-Like Cell Line, MOB 3–4, In Vitro
Department of Pharmacology, Niigata University School of Dentistry Niigata, Niigata 951
The effect of NaF on prostaglandin E2 (PGE2) synthesis in a clonal osteoblast-like cell line, MOB 3-4, was examined in the presence of Al3+. The MOB 3-4 cell line, which was derived from neonatal mouse calvaria, displays many osteoblastic characteristics, including the biosynthesis of PGE2. In the absence of Al3+, 1 mM NaF increased PGE2 synthesis (per well) to about 340% of the control level, whereas NaF at lower concentrations (below 0.1 mM) did not show such a significant effect. In the presence of 10 µM Al3+, NaF concentrations ranging from 0.01 to 1 mM increased PGE2 synthesis in a dose-dependent manner, though 10 µM Al3+ had no effect by itself. Similar effects were observed on alkaline phosphatase (ALP) activity per well, but a stimulatory effect of NaF on protein synthesis was observed only in the presence of 10µM Al3+. These data demonstrated that PGE2 synthesis per protein was increased by NaF alone, and this effect was markedly enhanced by the addition of AICI3. ALP activity per protein was, however, significantly increased by NaF in the absence of AICL3. Taken together with our previous finding that Al3+ enhances the NaF–, induced Ca2+ mobilization in MOB 3-4 cells, these results suggest that F– combined with Al3+ (i.e., AlF4–) is a more potent stimulator of PGE2 synthesis in cells than F– alone, and that the AIF4– -enhanced PGE2 synthesis may be caused by an increase in cytosolic free Ca2+ concentration during activation of the G protein by AIF4–.