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J. Biochem, 1989, Vol. 106, No. 1 98-103
© 1989 Japanese Biochemical Society

research-article

Structure and Expression of Mouse Apolipoprotein E Grene1

Kenji Horiuchi2, Shoji Tajima, Masakazu Menju and Akira Yamamoto

Department of Etiology and Pathophysiology, National Cardiovascular Center Research Institute Suita, Osaka 565

The mouse apolipoprotein E gene was isolated from a genomic library by screening with a cDNA probe. DNA including apolipoprotein E gene plus segments 2.5 kilobases upstream and 0.3 kilobase downstream of the coding region was transfected into NIH3T3 cells. The cells expressed the same-size apolipoprotein E mRNA and protein as those produced by mouse endogenously. The nucleotide sequence of the gene plus 5' and 3'flanking regions (one kilobase each) was determined. The sequence of the mouse apolipoprotein E gene was highly homologous to that of the rat gene, not only in the coding regions but also in the non-coding and intron regions. The mouse and the human apolipoprotein E genes were homologous in the 5’ proximal flanking region up to about 200 nucleotides as well as in the four exons. This proximal region was highly conserved for the genes of mouse, rat and human; the relative positions of the "TATA box" and the two copies of "GC box" were identical.

2K.H. is a research trainee form the Institute of Life Science Research, Nihon Nohyaku Co., Ltd.

1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Health and Welfare of Japan.


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