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J. Biochem, 1990, Vol. 107, No. 5 740-742
© 1990 Japanese Biochemical Society

research-article

Induction of GD3 Ganglioside by Adenovirus E1A Gene 13S- and 12S-mRNA Products in Rat 3Y1 Cells1

Yutaka Sanai*, Kinichiro Oda** and Yoshitaka Nagai*

*Department of Biochemistry, Faculty of Medicine, The University of Tokyo Tokyo 113
**Department of Biotechnology, Faculty of Basic Technology, Science University of Tokyo Noda, Chiba 278
* Department of Biochemistry, Faculty of Medicine, The University of Tokyo Tokyo 113

The effect of the expression of the human adenovirus type 12 E1A gene on ganglioside composition of rat 3Y1 cells was investigated using cell lines established by introduction of recombinant vector DNA containing the E1A 13S- or 12S-mRNA cDNA placed downstream of the hormone-inducible promoter of mouse mammary tumor virus. Ganglioside GD3 was newly detected after switching on of either 13S- or 12S-mRNA cDNA by addition of dexamethasone. A kinetic study indicated that GD3 was expressed 8 h after additon of dexamethasone. Gangloside GM2 was also accumulated by induction of 13S-mRNA of adenovirus E1A gene. The results indicated that 13S- or 12S-mRNA product alone has the ability to induce GD3 ganglioside in rat 3Y1 cells.

1 This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. The study was also supported by a grant from Yamanouchi Foundation for Research on Metabolic Disorders.


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