Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Ohmori, E.
Right arrow Articles by Ichikawa, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohmori, E.
Right arrow Articles by Ichikawa, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1990, Vol. 107, No. 6 834-839
© 1990 Japanese Biochemical Society

research-article

Purification and Characterization of L-Histidine Decarboxylase from Mouse Mastocytoma P-815 Cells

Eiji Ohmori, Tetsuya Fukui, Noriaki Imanishi, Kimio Yatsunami and Atsushi Ichikawa

Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606

Histidine decarboxylase was purified from mouse mastocytoma P–815 cells to electropho-retic homogeneity by ammonium sulfate fractionation, dialyses at pH 7.5 and 6.0, chroma-tographies on DEAE-Sepharose CL-6B, Phenyl-Sepharose CL-4B and Hydroxylapatite, Phenyl-Superose HPLC, Mono Q HPLC, and Diol-200 gel filtration HPLC. Under the assay conditions used, the pure enzyme exhibited a specific activity of 800 nmol/min/mg, which constituted 12,600-fold purification compared to the crude extract, with a 7% yield. The two-step dialysis turned out to be essential for removing the factor(s) which interfered with the enzyme purification. The optimum pH for the enzyme reaction was 6.6 and the isoelectric point of the enzyme was pH 5.4. The molecular mass of the enzyme was found to be approximately 53 kDa on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, 110 kDa on gel filtration, and 115 kDa on polyacrylamide gradient gel electrophoresis in the absence of sodium dodecyl sulfate. The Km value for histidine was estimated to be 0.26 mM at pH 6.8.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Furuta, K. Nakayama, Y. Sugimoto, A. Ichikawa, and S. Tanaka
Activation of Histidine Decarboxylase through Post-translational Cleavage by Caspase-9 in a Mouse Mastocytoma P-815
J. Biol. Chem., May 4, 2007; 282(18): 13438 - 13446.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. V. Fleming and T. C. Wang
The Production of 53-55-kDa Isoforms Is Not Required for Rat L-Histidine Decarboxylase Activity
J. Biol. Chem., January 3, 2003; 278(1): 686 - 694.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Tanaka, Y. Takasu, S. Mikura, N. Satoh, and A. Ichikawa
Antigen-independent Induction of Histamine Synthesis by Immunoglobulin E in Mouse Bone Marrow-derived Mast Cells
J. Exp. Med., July 15, 2002; 196(2): 229 - 235.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Safina, S. Tanaka, M. Inagaki, K. Tsuboi, Y. Sugimoto, and A. Ichikawa
Expression of L-Histidine Decarboxylase in Mouse Male Germ Cells
J. Biol. Chem., April 12, 2002; 277(16): 14211 - 14215.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Tanaka, K.-i. Nemoto, E. Yamamura, and A. Ichikawa
Intracellular Localization of the 74- and 53-kDa Forms of L-Histidine Decarboxylase in a Rat Basophilic/Mast Cell Line, RBL-2H3
J. Biol. Chem., April 3, 1998; 273(14): 8177 - 8182.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Ohtsu, A. Kuramasu, S. Suzuki, K. Igarashi, Y. Ohuchi, M. Sato, S. Tanaka, S. Nakagawa, K. Shirato, M. Yamamoto, et al.
Histidine Decarboxylase Expression in Mouse Mast Cell Line P815 Is Induced by Mouse Peritoneal Cavity Incubation
J. Biol. Chem., November 8, 1996; 271(45): 28439 - 28444.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.