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J. Biochem, 1990, Vol. 108, No. 1 122-127
© 1990 Japanese Biochemical Society

research-article

Steroid Transhydrogenase Activity of 3-Ketosteroid-{delta}-Dehydrogenase from Nocardia corallina

Eiji Itagaki1, Hiroyuki Matushita and Tadayuki Hatta

Department of Chemistry, Faculty of Science, Kanazawa University Kanazawa, Ishikawa 920

1To whom correspondence should be addressed

3-Ketosteroid-{Delta}1 -dehydrogenase from Nocardia corallina catalyzes transhydrogenation of 3-keto-4-ene-steroid to 3-keto-l,4-diene-steroid e.g., progesterone to 1,4-androstadiene-3,17-dione. The reaction proceeded linearly at first and then soon slowed down owing to equilibration. The turnover number of this reaction was of the same magnitude as that of the dehydrogenation of 3-keto-4-ene-steroid. The pH optimum was 8.4, which is lower than that of the dehydrogenase reaction. The enzyme has a wide specificity for hydrogen acceptor steroids. The Km' and KMax' values for these steroids and the values of the corresponding 3-keto-4-ene-steroids were compared. Kinetic studies of the steroid transhydrogenase reaction demonstrated a typical ping-pong mechanism. The enzyme oxidized 1,2-tritiated progesterone and transferred the tritium atoms to the reaction product, 4-androstene-3,17-dione, and water. Transhydrogenation in D2O resulted in the incorporation of a deuterium atom into the C2-position of 4-androstene-3,17-dione. The results indicate that the enzyme catalyzes C1,C2-trans axial abstraction of hydrogen atoms from progesterone, transfer of the la-hydrogen to the C1-position of l,4-androstadiene-3,17-dione and release of the 2ß-hydrogen to water. Reaction schemes based on the experimental results are proposed. The enzyme also catalyzes the reduction of 3-keto-l,4-diene-steroids with reduced benzyl viologen.


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