Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Yoshlda, M.
Right arrow Articles by Nakamura, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshlda, M.
Right arrow Articles by Nakamura, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1990, Vol. 108, No. 2 158-165
© 1990 Japanese Biochemical Society

research-article

Three-Dimensional Structure of an {alpha}-Amylase Inhibitor HAIM as Determined by Nuclear Magnetic Resonance Methods

Mayumi Yoshlda*, Takahisa Nakai*, Kenichi Fukuhara*, Shin Saitoh*, Wataru Yoshikawa*,1, Yuji Kobayashi** and Haruki Nakamura*

*Protein Engineering Research Institute Suita, Osaka 565
**Institute for Proyein Research, Osaka University Suita, Osaka 565

1To whom correspondence should be addressed

The three-dimensional structure of an {alpha}-amylase inhibitor, HAIM, composed of 78 amino acids, was analyzed by two-dimensional NMR techniques. Sequence-specific assignments were made for the amino acid residues from Iie-6 to Cys-72. Distance geometry analysis of the Interresldue NOEs revealed that the HAIM molecule consists of two ß-sheets, as Is the case In a homologous {alpha}-aniylase Inhibitor, Tendamistat, though one of its ß-strands is much shorter than that of Tendamistat. The combination of molecular modeling from Tendamistat and distance geometry analysis was confirmed to be useful for our purpose.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.