Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Kubo, H.
Right arrow Articles by Hoshi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kubo, H.
Right arrow Articles by Hoshi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1990, Vol. 108, No. 2 185-192
© 1990 Japanese Biochemical Society

research-article

Gangliosides from the Eggs of the Sea Urchin, Anthocidaris crassispina1

Hideo Kubo*,**, Atsushi Irie*, Fuyuhiko Inagaki*** and Motonori Hoshi*,2

*Department of Life Science, Faculty of Science, Tokyo Institute of Technology Meguro-ku, Tokyo 152
**Developmental and Reproductive Biology Center Yainagata, Yamagata 990
***Department of Molecular Physiology, The Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113

2To whom correspondence should be addressed

NeuGc{alpha}2-6Glcß1-1Cer (M5 ganglioside) and HSO2-8NeuGc{alpha}2-6Glcß1-1Cer (T1 gangilo side) were purified by column chromatographies with DEAE-Sephadex A-25 and silicic acid from the eggs of the sea urchin, Anthocidaris crassispina. Their chemical structures were determined by gas-liquid chromatography, methylation analysis, enzymatic hydrol ysis, negative-ion fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance spectroscopy. Long-chain base compositions of both gangliosides were almost identical: all the long-chain bases were phytosphingosines, and C18-phytosphingosine accounted for more than 95% of them. Fatty acid compositions were also very similar: the main fatty acids were 22:1, 23:1, 24:1, and their 2-hydroxylated forms, and the 2-hydroxy fatty acids amounted to 65.3 and 74.3% of the fatty acids in M5 and T1 gangliosides, respectively. Proton nuclear magnetic resonance spectroscopic study revealed a down field-shifted H8 proton signal of NeuGc residue in T1 ganglioside, in agreement with the presence of sulfate ester at the 08 position.

1This work was supported in part by Grants-in-Aid for Co-operative Research (No. 61304034), General Scientific Research (No. 61440006), and Scientific Research on Priority Areas (No. 63640003) from the Ministry of Education, Science and Culture of Japan.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
M. Ito, Y. Matsumuro, S. Yamada, T. Kitamura, S. Itonori, and M. Sugita
Isolation and characterization of a novel uronic acid-containing acidic glycosphingolipid from the ascidian Halocynthia roretzi
J. Lipid Res., January 1, 2007; 48(1): 96 - 103.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
S. Miyata, C. Sato, S. Kitamura, M. Toriyama, and K. Kitajima
A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an {alpha}2,9-linked poly-N-acetylneuraminic acid chain, capped by an 8-O-sulfated sialic acid residue
Glycobiology, September 1, 2004; 14(9): 827 - 840.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
M. Nezuo, H. Shogomori, M. Hoshi, T. Yamamoto, T. Teshima, T. Shiba, and K. Chiba
Developmental changes in localization of the main ganglioside during sea urchin embryogenesis
Glycobiology, November 1, 2000; 10(11): 1243 - 1247.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kitazume, K. Kitajima, S. Inoue, S. M. Haslam, H. R. Morris, A. Dell, W. J. Lennarz, and Y. Inoue
The Occurrence of Novel 9-O-Sulfated N-Glycolylneuraminic Acid-capped alpha2[IMAGE]5-O[IMAGE]-linked Oligo/PolyNeu5Gc Chains in Sea Urchin Egg Cell Surface Glycoprotein
J. Biol. Chem., March 22, 1996; 271(12): 6694 - 6701.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.