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J. Biochem, 1990, Vol. 108, No. 2 200-206
© 1990 Japanese Biochemical Society

research-article

Characterization of Gene Organization and Generation of Heterogeneous mRNA Species of Rat ISK Protein1

Masazumi Iwai*,**, Masayuki Masu*, Kunihiro Tsuchida*, Takahide Mori**, Hiroaki Ohkubo* and Shigetada Nakanishi*

*Institute for Inununology, Kyoto University Faculty of Medicine Sakyo-ku, Kyoto, Kyoto 606
**Department of Gynecology and Obstetrics, Kyoto University Faculty of Medicine Sakyo-ku, Kyoto, Kyoto 606

The ISK protein is a novel, probably epithelial potassium channel which differs from conventional potassium channels in its structure, electrophysiology, and tissue distribu tion. In this investigation, we isolated and analyzed genomic and cDNA clones coding for the rat ISK protein to characterize the structural organization and expression pattern of the ISK protein gene. This analysis, together with primer extension and RNase protection experiments, indicated that the ISK protein mRNA is initiated from two different upstream exons and then encoded by an uninterrupted downstream exon covering the protein-coding and the 3'-untranslated regions of the mRNA. RNA blot hybridization analysis showed additional generation of several large species of mRNAs which result from Inclusion of a part of the intron sequence and the 3'-flanking region of the ISK protein gene. Thus, the single ISK protein gene is involved in the production of multiple species of mRNAs through a variety of cellular mechanisms including transcription initiation at different sites, alterna tive RNA splicing, and polyadenylation at different sites. The heterogeneity of the ISK protein mRNM may be associated with the emergence of the functional and regulatory diversity observed for potassium ion permeation in epithelial cells.

1This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Institute of Physical and Chemical Research, and the Science and Technology Agency of Japan.


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