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J. Biochem, 1990, Vol. 108, No. 2 292-296
© 1990 Japanese Biochemical Society

research-article

Studies on the Superoxide Releasing Site in Plasma Membranes of Neutrophils with ESR Spin-Labels

Hirotada Fujii and Katsuko Kakinuma

The Tokyo Metropolitan Institate of Medical Science Bunkyo-ku, Thkyo 113

Superoxide (O2–) membranes of pig blood neutrophils were studied by the ESR spin-label method. Neutrophils were spin-labeled with doxylstearic acids, consisting of nitroxide free radicals bonded to the 5, 7, 12, or 16 position of stearic acid (5-, 7-, 12-, or 16-DS), to detect the reduction of their nitroxide radicals at different positions in the membrane. The spin-labeled cells were then stimulated with phorbol myristate acetate (PMA). Stimulation of the labeled cells resulted in a marked decrease in the spin concentra tion of 5-DS due to the reduction by O2–, but not in those of the other three DS labels. This reduction of 5-DS was completely inhibited by copper salicylate (CS), a hydrophobic and permeable O2–-scavenger, but not by superoxide dismutase (SOD). CS was not inhibitory on the respiratory burst, i.e., O2–-generating activity of neutrophils. On the contrary, if the spin-labels were present in the extracellular medium, SOD inhibited the reduction of all four D8 labels due to O2– released from PMA-stimulated cells. These results suggest that the O2– -releasing site is not located at the outer surface of the plasma membrane but in an inner hydrophobic environment a short distance (around 4-5 A) from its outer surface.


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