J. Biochem, 1990, Vol. 108, No. 2 297-302
© 1990 Japanese Biochemical Society
research-article |
A Heparin Binding Protein Whose Expression Increases during Differentiation of Embryonal Carcinoma Cells to Parietal Endoderm Cells: cDNA Cloning and Sequence Analysis1
Department of Biochemistry, Faculty of Medicine, Kagoshisna University Kagoshirno, Kagoshkna 890
A cDNA clone isolated from a 
gtll expression library of teratocarcinoma OTTBO5O specifies for a glycoprotein with a molecular weight of about 44,000. The new glycoprotein was termed heparin binding protien -44 (HIBP-44), since It was absorbed to a heparin agarose column and was eluted from it by a buffer containIng 1.5 M NaC1. HLBP-44 mRNA was intensely expressed in PYS-2 parietal endoderm cells and in the kidney, and the RNA level Increased about 10-fold during differentiation of FL) embryonal carcinoma cells to parietal endoderm cells. From the eDNA sequence, IIBP-44 was concluded to be rich in charged amino acids, and large segments of the protein appeared to form 
-helixes. The protein was considered to be anchored to the membrane by a cluster of hydrophobic amino acids present in the N-terminal region. Indeed, the N-terminal sequence of HBP-44 was homologous to asialoglycoprotein receptor, which Is anchored to the membrane by the N-terminal region. Furthermore, a portion of the N-terminal region of HIBP-44 was homologous to the leucine zipper domain. Except for the N-terminal region, HBP-44 had over-all homology with structural proteins such as myosin heavy chain. We propose that BIBP-44 is extruded from plasma membranes and interacts with heparin and related molecules and that It is Involved in the interactions of plasma membranes with basement membranes.
1This work was supported by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Verges, A. Bensadoun, J. Herz, J. D. Belcher, and R. J. Havel Endocytosis of Hepatic Lipase and Lipoprotein Lipase into Rat Liver Hepatocytes in Vivo Is Mediated by the Low Density Lipoprotein Receptor-related Protein J. Biol. Chem., March 5, 2004; 279(10): 9030 - 9036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. BIRN, H. VORUM, P. J. VERROUST, S. K. MOESTRUP, and E. I. CHRISTENSEN Receptor-Associated Protein Is Important for Normal Processing of Megalin in Kidney Proximal Tubules J. Am. Soc. Nephrol., February 1, 2000; 11(2): 191 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Umans, L. Overbergh, L. Serneels, I. Tesseur, and F. Van Leuven Analysis of Expression of Genes Involved in Apolipoprotein E-Based Lipoprotein Metabolism in Pregnant Mice Deficient in the Receptor-Associated Protein, the Low Density Lipoprotein Receptor, or Apolipoprotein E Biol Reprod, November 1, 1999; 61(5): 1216 - 1225. [Abstract] [Full Text]
|
|
|
|
|
|
L. V. Medved, M. Migliorini, I. Mikhailenko, L. G. Barrientos, M. Llinas, and D. K. Strickland Domain Organization of the 39-kDa Receptor-associated Protein J. Biol. Chem., January 8, 1999; 274(2): 717 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Mikhailenko, D. Krylov, K. M. Argraves, D. D. Roberts, G. Liau, and D. K. Strickland Cellular Internalization and Degradation of Thrombospondin-1 Is Mediated by the Amino-terminal Heparin Binding Domain (HBD). HIGH AFFINITY INTERACTION OF DIMERIC HBD WITH THE LOW DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN J. Biol. Chem., March 7, 1997; 272(10): 6784 - 6791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Nielsen, A. Nykjær, I. Warshawsky, A. L. Schwartz, and Jør. Gliemann Analysis of Ligand Binding to the alpha(2)-Macroglobulin Receptor/Low Density Lipoprotein Receptor-related Protein J. Biol. Chem., October 6, 1995; 270(40): 23713 - 23719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Umans, L. Serneels, L. Overbergh, K. Lorent, F. Van Leuven, and H. Van den Berghe Targeted Inactivation of the Mouse alpha(2)-Macroglobulin Gene J. Biol. Chem., August 25, 1995; 270(34): 19778 - 19785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Stefansson, D. A. Chappell, K. M. Argraves, D. K. Strickland, and W. S. Argraves Glycoprotein 330/Low Density Lipoprotein Receptor-related Protein-2 Mediates Endocytosis of Low Density Lipoproteins via Interaction with Apolipoprotein B100 J. Biol. Chem., August 18, 1995; 270(33): 19417 - 19421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Grammatikakis, A. Grammatikakis, M. Yoneda, Q. Yu, S. D. Banerjee, and B. P. Toole A Novel Glycosaminoglycan-binding Protein Is the Vertebrate Homologue of the Cell Cycle Control Protein, Cdc37 J. Biol. Chem., July 7, 1995; 270(27): 16198 - 16205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Xu, Y. Takahashi, T. Sakashita, T. Iwasaki, H. Hattori, and T. Yoshimoto Low Density Lipoprotein Receptor-related Protein Is Required for Macrophage-mediated Oxidation of Low Density Lipoprotein by 12/15-Lipoxygenase J. Biol. Chem., September 21, 2001; 276(39): 36454 - 36459. [Abstract] [Full Text] [PDF]
|
|